Applications in biology research, such as electrophysiology, require micrometer precision cutting to isolate thin slices of tissue for cell analysis and sample characterization. This challenging operation is difficult to achieve with traditional manual methods, such as using sharp scalpels, because dissociating the layers by hand is insufficiently precise.
In this experiment, two miBots micromanipulators were used for the micro-dissection of a 500 µm wide and 3 mm long mouse brain slice under a Leica MZ16 FA stereomicroscope. One miBot was used to cut the tissue and the other one to gently separate the cut portion from the rest of the slice.
The above figures illustrate the cut progression of a 300 µm thick parasagittal slice of the rodent brain.
Made in collaboration with
Laboratory of Neural Microcircuitry, Brain Mind Institute, EPFL, Lausanne, Switzerland (http://markram-lab.epfl.ch)